Composite

Part:BBa_K1919101:Design

Designed by: Zekun Lv   Group: iGEM16_SCU-China   (2016-10-14)


A temperature promoter cotaining clts repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we choose five important sites A44G, G92A, C188T, G378A, A629T to mutant.


Source

It is synthtetised by Tsingke.

References